Repurposing agricultural waste as low-cost cultured meat scaffolds

Growing meat in vitro using tissue engineering and bioproduction techniques (cellular agriculture) has become an increasingly promising solution to the global food security challenge. Our lab established methods cultivate bovine muscle on decellularized plants, representing a viable low-cost, sustainable method grow edible scaffolds. Most work this area focused use of plant materials (i.e., spinach leaves, apple, broccoli) with inherent economic value. Harvest waste such as corn husk or jackfruit represent abundant sources cellulose for scaffold production may be alternative. The present study aims investigate cultured through decellularized, samples rind. Corn husks rinds were exposed immersion decellularization. DNA quantification histological analysis demonstrated sufficient decellularization (0.17 ± 0.06 0.07 0.00 ug DNA/g rinds, respectively). Following decellularization, stiffnesses decreased from 56.67±16.71 MPa 12.95±2.43 fiber-aligned direction, while 7.54 ±2.42 2.47±1.47 MPa. Seeded scaffolds satellite cells (BSCs) (11.45±2.24 ug/ul lysate/Gram) avian (QM7s) (12.90±1.99 increased protein yields QM7 yielded but PBSCs seeded did not yield content higher than controls (QM7 husk: 16.28±3.55, husks: 9.57±1.56 lysate/Gram, control: 6.35±1.43 lysate/Gram). Additionally, cell transfer (bead-to-bead transfer) was observed dynamic environment. These results suggest that harvest aid realization products will contribute more robust environmentally supply.